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GenScript corporation brainbow cassette

The <t>Brainbow</t> design, and recombination events that can support expression of each FP. (A) The Brainbow transcription unit, as constructed in E. coli, before Cre-mediated recombination; the initial order of the FP genes. The Brainbow transcription unit contains the VGLUT1 promoter (black segment with arrow), loxP sites (black triangles), and four FP genes (EmGFP (Tsien, 1998), green; mOrange2 (Shaner et al., 2008), yellow; LSSmKate2 (Piatkevich et al., 2010), red; or EBFP2 (Ai et al., 2007), blue); the orientation of each gene is indicated by a white arrow; and each gene is followed by a unique polyadenylation site (A1+ to A4+). Cre-mediated recombination between loxP sites in an inverted orientation leads to inversion, which is reversible; Cre-mediated recombination between loxP sites in the same orientation leads to deletion, which is irreversible. (B) Inversions can support expression of each FP gene. The first line shows the product of an inversion between the first and second loxP sites. The second line shows the product of an inversion between the first and fourth loxP sites; and the third line shows the product of a subsequent inversion between the first and second loxP sites. (C) A deletion followed by an inversion can support expression of each FP gene. The first line shows the product of a deletion between the second and fourth loxP sites in the initial orientation; the second line shows the product of a subsequent inversion. The third line shows the product of a deletion between the first and third loxP sites in the initial orientation; the fourth line shows the product of a subsequent inversion.

Brainbow Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brainbow cassette/product/GenScript corporation
Average 90 stars, based on 1 article reviews

brainbow cassette - by Bioz Stars, 2026-04

90/100 stars

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1) Product Images from "Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow"

Article Title: Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow

Journal: Journal of neuroscience methods

doi: 10.1016/j.jneumeth.2014.11.009

The Brainbow design, and recombination events that can support expression of each FP. (A) The Brainbow transcription unit, as constructed in E. coli, before Cre-mediated recombination; the initial order of the FP genes. The Brainbow transcription unit contains the VGLUT1 promoter (black segment with arrow), loxP sites (black triangles), and four FP genes (EmGFP (Tsien, 1998), green; mOrange2 (Shaner et al., 2008), yellow; LSSmKate2 (Piatkevich et al., 2010), red; or EBFP2 (Ai et al., 2007), blue); the orientation of each gene is indicated by a white arrow; and each gene is followed by a unique polyadenylation site (A1+ to A4+). Cre-mediated recombination between loxP sites in an inverted orientation leads to inversion, which is reversible; Cre-mediated recombination between loxP sites in the same orientation leads to deletion, which is irreversible. (B) Inversions can support expression of each FP gene. The first line shows the product of an inversion between the first and second loxP sites. The second line shows the product of an inversion between the first and fourth loxP sites; and the third line shows the product of a subsequent inversion between the first and second loxP sites. (C) A deletion followed by an inversion can support expression of each FP gene. The first line shows the product of a deletion between the second and fourth loxP sites in the initial orientation; the second line shows the product of a subsequent inversion. The third line shows the product of a deletion between the first and third loxP sites in the initial orientation; the fourth line shows the product of a subsequent inversion.

Figure Legend Snippet: The Brainbow design, and recombination events that can support expression of each FP. (A) The Brainbow transcription unit, as constructed in E. coli, before Cre-mediated recombination; the initial order of the FP genes. The Brainbow transcription unit contains the VGLUT1 promoter (black segment with arrow), loxP sites (black triangles), and four FP genes (EmGFP (Tsien, 1998), green; mOrange2 (Shaner et al., 2008), yellow; LSSmKate2 (Piatkevich et al., 2010), red; or EBFP2 (Ai et al., 2007), blue); the orientation of each gene is indicated by a white arrow; and each gene is followed by a unique polyadenylation site (A1+ to A4+). Cre-mediated recombination between loxP sites in an inverted orientation leads to inversion, which is reversible; Cre-mediated recombination between loxP sites in the same orientation leads to deletion, which is irreversible. (B) Inversions can support expression of each FP gene. The first line shows the product of an inversion between the first and second loxP sites. The second line shows the product of an inversion between the first and fourth loxP sites; and the third line shows the product of a subsequent inversion between the first and second loxP sites. (C) A deletion followed by an inversion can support expression of each FP gene. The first line shows the product of a deletion between the second and fourth loxP sites in the initial orientation; the second line shows the product of a subsequent inversion. The third line shows the product of a deletion between the first and third loxP sites in the initial orientation; the fourth line shows the product of a subsequent inversion.

Techniques Used: Expressing, Construct

The structure of pVGLUT1brainbow and the production of Brainbow arrays by rolling circle DNA replication. (A) A schematic diagram of pVGLUT1brainbow. The VGLUT1 promoter (wavy segment with arrow) supports expression of Brainbow (clear segment). The vector backbone contains an HSV-1 origin of DNA replication (oriS, black circle with white interior in the short line segment) and an HSV-1 a sequence (cross hatched segment), which contains the packaging site; these elements support replication and packaging of the vector, respectively. A cassette of three polyadenylation sites (tri-poly A, black segment) was placed 5′ to the VGLUT1 promoter to reduce any effects on expression from the HSV-1 immediate early 4/5 promoter (contained in short line segment). Sequences from pBR322 (diagonal line segment) support growth in E. coli. (B) Concatamers of the vector are produced during the second, rolling circle phase, of HSV-1 DNA replication (Roizman and Sears, 1993). A HSV-1 genome-sized concatamer of the vector is packaged into a HSV-1 particle (Fraefel et al., 1996; Roizman and Sears, 1993); the HSV-1 genome is ~152 kb, and pVGLUT1brainbow is ~20 kb; thus, an array of 7 or 8 pVGLUT1brainbow is packaged into a HSV-1 particle.

Figure Legend Snippet: The structure of pVGLUT1brainbow and the production of Brainbow arrays by rolling circle DNA replication. (A) A schematic diagram of pVGLUT1brainbow. The VGLUT1 promoter (wavy segment with arrow) supports expression of Brainbow (clear segment). The vector backbone contains an HSV-1 origin of DNA replication (oriS, black circle with white interior in the short line segment) and an HSV-1 a sequence (cross hatched segment), which contains the packaging site; these elements support replication and packaging of the vector, respectively. A cassette of three polyadenylation sites (tri-poly A, black segment) was placed 5′ to the VGLUT1 promoter to reduce any effects on expression from the HSV-1 immediate early 4/5 promoter (contained in short line segment). Sequences from pBR322 (diagonal line segment) support growth in E. coli. (B) Concatamers of the vector are produced during the second, rolling circle phase, of HSV-1 DNA replication (Roizman and Sears, 1993). A HSV-1 genome-sized concatamer of the vector is packaged into a HSV-1 particle (Fraefel et al., 1996; Roizman and Sears, 1993); the HSV-1 genome is ~152 kb, and pVGLUT1brainbow is ~20 kb; thus, an array of 7 or 8 pVGLUT1brainbow is packaged into a HSV-1 particle.

Techniques Used: Expressing, Plasmid Preparation, Sequencing, Produced

pVGLUT1brainbow supports labeling of POR cortex neurons and axons, and distant axons in PER cortex, with different hues. Rats were sacrificed at 4 days (A–E) or 8 days (F) after gene transfer, brains were sectioned, and confocal stacks were analyzed. (A) A brightfield, low power view of POR cortex in a section proximal to the injection site. The arrow indicated the rhinal sulcus. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A high power view of the boxed area from (B). Neurons and proximal axons that contain different hues are shown. Arrows, cell bodies; arrowheads, axons. (D and E) High power views of neurons and proximal axons, in POR cortex, that contain different hues, from different sections. Some of the labeled cell bodies contain proximal processes, particularly in (D). Also, some axons that are not connected to a cell body are visible. (F) A high power view of PER cortex that shows one axon for ~50 um in length (arrow) and other axons in cross section (arrowheads). Scale bars: (A and B) 250 μm, (C–E) 50 μm, (F) 50 μm.

Figure Legend Snippet: pVGLUT1brainbow supports labeling of POR cortex neurons and axons, and distant axons in PER cortex, with different hues. Rats were sacrificed at 4 days (A–E) or 8 days (F) after gene transfer, brains were sectioned, and confocal stacks were analyzed. (A) A brightfield, low power view of POR cortex in a section proximal to the injection site. The arrow indicated the rhinal sulcus. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A high power view of the boxed area from (B). Neurons and proximal axons that contain different hues are shown. Arrows, cell bodies; arrowheads, axons. (D and E) High power views of neurons and proximal axons, in POR cortex, that contain different hues, from different sections. Some of the labeled cell bodies contain proximal processes, particularly in (D). Also, some axons that are not connected to a cell body are visible. (F) A high power view of PER cortex that shows one axon for ~50 um in length (arrow) and other axons in cross section (arrowheads). Scale bars: (A and B) 250 μm, (C–E) 50 μm, (F) 50 μm.

Techniques Used: Labeling, Injection

pVGLUT1brainbow can label neurons and proximal axons in the hippocampal dentate gyrus with different hues. Rats were sacrificed at 4 days after gene transfer, and confocal stacks were collected. (A) A brightfield, low power view of the hippocampus and adjacent areas in a section proximal to the injection site. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A medium power view of the boxed area from (B) showing Brainbow labeled cells. Arrows, cell bodies. (D) A high power view of the boxed area from (C) showing Brainbow labeled neurons and proximal axons. Arrows, cell bodies; arrowheads, axons. (E and F) High power views of Brainbow labeled neurons and proximal axons from different sections that contain the dentate gyrus. Some of the labeled cell bodies contain proximal processes, particularly a blue neuron on the left side of (E) contains an axon that extends for ~50 μm. Also, some axons that are not connected to a cell body are visible. Scale bars: (A) 500 μm, (B) 250 μm, (C) 100 μm, (D–F) 50 μm.

Figure Legend Snippet: pVGLUT1brainbow can label neurons and proximal axons in the hippocampal dentate gyrus with different hues. Rats were sacrificed at 4 days after gene transfer, and confocal stacks were collected. (A) A brightfield, low power view of the hippocampus and adjacent areas in a section proximal to the injection site. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A medium power view of the boxed area from (B) showing Brainbow labeled cells. Arrows, cell bodies. (D) A high power view of the boxed area from (C) showing Brainbow labeled neurons and proximal axons. Arrows, cell bodies; arrowheads, axons. (E and F) High power views of Brainbow labeled neurons and proximal axons from different sections that contain the dentate gyrus. Some of the labeled cell bodies contain proximal processes, particularly a blue neuron on the left side of (E) contains an axon that extends for ~50 μm. Also, some axons that are not connected to a cell body are visible. Scale bars: (A) 500 μm, (B) 250 μm, (C) 100 μm, (D–F) 50 μm.

Techniques Used: Injection, Labeling

Image Search Results

Journal: Journal of neuroscience methods

Article Title: Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow

doi: 10.1016/j.jneumeth.2014.11.009

Figure Lengend Snippet: The Brainbow design, and recombination events that can support expression of each FP. (A) The Brainbow transcription unit, as constructed in E. coli, before Cre-mediated recombination; the initial order of the FP genes. The Brainbow transcription unit contains the VGLUT1 promoter (black segment with arrow), loxP sites (black triangles), and four FP genes (EmGFP (Tsien, 1998), green; mOrange2 (Shaner et al., 2008), yellow; LSSmKate2 (Piatkevich et al., 2010), red; or EBFP2 (Ai et al., 2007), blue); the orientation of each gene is indicated by a white arrow; and each gene is followed by a unique polyadenylation site (A1+ to A4+). Cre-mediated recombination between loxP sites in an inverted orientation leads to inversion, which is reversible; Cre-mediated recombination between loxP sites in the same orientation leads to deletion, which is irreversible. (B) Inversions can support expression of each FP gene. The first line shows the product of an inversion between the first and second loxP sites. The second line shows the product of an inversion between the first and fourth loxP sites; and the third line shows the product of a subsequent inversion between the first and second loxP sites. (C) A deletion followed by an inversion can support expression of each FP gene. The first line shows the product of a deletion between the second and fourth loxP sites in the initial orientation; the second line shows the product of a subsequent inversion. The third line shows the product of a deletion between the first and third loxP sites in the initial orientation; the fourth line shows the product of a subsequent inversion.

Article Snippet: The Brainbow cassette was synthesized (Genscript) as four fragments, each in pUC57, and then assembled.

Techniques: Expressing, Construct

Journal: Journal of neuroscience methods

Article Title: Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow

doi: 10.1016/j.jneumeth.2014.11.009

Figure Lengend Snippet: The structure of pVGLUT1brainbow and the production of Brainbow arrays by rolling circle DNA replication. (A) A schematic diagram of pVGLUT1brainbow. The VGLUT1 promoter (wavy segment with arrow) supports expression of Brainbow (clear segment). The vector backbone contains an HSV-1 origin of DNA replication (oriS, black circle with white interior in the short line segment) and an HSV-1 a sequence (cross hatched segment), which contains the packaging site; these elements support replication and packaging of the vector, respectively. A cassette of three polyadenylation sites (tri-poly A, black segment) was placed 5′ to the VGLUT1 promoter to reduce any effects on expression from the HSV-1 immediate early 4/5 promoter (contained in short line segment). Sequences from pBR322 (diagonal line segment) support growth in E. coli. (B) Concatamers of the vector are produced during the second, rolling circle phase, of HSV-1 DNA replication (Roizman and Sears, 1993). A HSV-1 genome-sized concatamer of the vector is packaged into a HSV-1 particle (Fraefel et al., 1996; Roizman and Sears, 1993); the HSV-1 genome is ~152 kb, and pVGLUT1brainbow is ~20 kb; thus, an array of 7 or 8 pVGLUT1brainbow is packaged into a HSV-1 particle.

Article Snippet: The Brainbow cassette was synthesized (Genscript) as four fragments, each in pUC57, and then assembled.

Techniques: Expressing, Plasmid Preparation, Sequencing, Produced

Journal: Journal of neuroscience methods

Article Title: Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow

doi: 10.1016/j.jneumeth.2014.11.009

Figure Lengend Snippet: pVGLUT1brainbow supports labeling of POR cortex neurons and axons, and distant axons in PER cortex, with different hues. Rats were sacrificed at 4 days (A–E) or 8 days (F) after gene transfer, brains were sectioned, and confocal stacks were analyzed. (A) A brightfield, low power view of POR cortex in a section proximal to the injection site. The arrow indicated the rhinal sulcus. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A high power view of the boxed area from (B). Neurons and proximal axons that contain different hues are shown. Arrows, cell bodies; arrowheads, axons. (D and E) High power views of neurons and proximal axons, in POR cortex, that contain different hues, from different sections. Some of the labeled cell bodies contain proximal processes, particularly in (D). Also, some axons that are not connected to a cell body are visible. (F) A high power view of PER cortex that shows one axon for ~50 um in length (arrow) and other axons in cross section (arrowheads). Scale bars: (A and B) 250 μm, (C–E) 50 μm, (F) 50 μm.

Article Snippet: The Brainbow cassette was synthesized (Genscript) as four fragments, each in pUC57, and then assembled.

Techniques: Labeling, Injection

Journal: Journal of neuroscience methods

Article Title: Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow

doi: 10.1016/j.jneumeth.2014.11.009

Figure Lengend Snippet: pVGLUT1brainbow can label neurons and proximal axons in the hippocampal dentate gyrus with different hues. Rats were sacrificed at 4 days after gene transfer, and confocal stacks were collected. (A) A brightfield, low power view of the hippocampus and adjacent areas in a section proximal to the injection site. (B) A low power view of Brainbow labeled neurons in the same section as in (A). (C) A medium power view of the boxed area from (B) showing Brainbow labeled cells. Arrows, cell bodies. (D) A high power view of the boxed area from (C) showing Brainbow labeled neurons and proximal axons. Arrows, cell bodies; arrowheads, axons. (E and F) High power views of Brainbow labeled neurons and proximal axons from different sections that contain the dentate gyrus. Some of the labeled cell bodies contain proximal processes, particularly a blue neuron on the left side of (E) contains an axon that extends for ~50 μm. Also, some axons that are not connected to a cell body are visible. Scale bars: (A) 500 μm, (B) 250 μm, (C) 100 μm, (D–F) 50 μm.

Article Snippet: The Brainbow cassette was synthesized (Genscript) as four fragments, each in pUC57, and then assembled.

Techniques: Injection, Labeling

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